EC Number   |
Reaction |
Reference |
|---|
   3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
Asp44 is involved in catalysis, while Met118, Gln134, Glu190, and Met236 are not |
650419 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
catalytic mechanism, the N-terminal 62 residues and the C-terminal 39 residues have important roles in 3'-exonuclease activity, residues 63-453 are responsible for 5'- and 3'-exonuclease activities, residues 24-492 form a region with 7fold higher catalytic activity than the intact enzyme |
649681 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
conserved residues Glu79, His135, Asp213, and His 252 are involved in catalysis |
651802 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
Glu128 is required for activity, contains conserved sequence LEDFXR, endonuclease activity |
649684 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
preference for double-stranded DNA |
650325, 650419, 651811, 653044, 653799 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
preference for double-stranded DNA. Similar enzymes: streptococcal DNase (streptodornase), T4 endonuclease II, T7 endonuclease II, E. coli endonuclease I, "nicking" nuclease of calf thymus, colicin E2 and E3, formerly EC 3.1.4.5 |
- |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
preference for single-stranded DNA |
651509 |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products |
preference for single-stranded DNA and 3'AMP, guanylic acid preferential endo-exonuclease, mode of action, overview |
651077 |
