EC Number   |
Reaction |
Reference |
|---|
    2.7.7.9 | UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose |
mechanism |
643748 |
| 2.7.7.9 | UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose |
ordered bi-bi mechanism. The molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate |
674571 |
| 2.7.7.9 | UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose |
ordered sequential Bi Bi mechanism, overview. Pyrimidine bases may approach the base binding site, but they cannot form the tight interactions observed in the HpUGPase/UDP-Glc complex. Thymine binding would be hindered by its extra methyl group, and cytosine could not make specific hydrogen bonds |
723188 |
| 2.7.7.9 | UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose |
sequential ordered bi bi reaction mechanism |
643726, 643743, 643752 |
| 2.7.7.9 | UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose |
Trp333 and Arg391 are essential for activity, while His266, Arg389, Arg422, Arg 445, and Trp218 are not |
643751 |
