EC Number |
Reaction |
Reference |
---|
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
also acts slowly with CTP, catalyses template-dependent extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de nove, the primer, depending on source of the enzyme, may be an RNA or DNA fragment, or oligo(a) bearing a 3'-OH terminal group, see also EC 2.7.7.6 DNA-directed RNA polymerase |
- |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
Arabidopsis possesses 4 genes for PAP, these genes are expressed at the level of mRNA in tissue-specific ways, transcripts from the four genes are alternatively spliced so as to yield mRNAs encoding highly truncated polypeptides |
661237 |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
binding of enzyme to nuclear poly(A) binding protein results in 80-fold increase in apparent affinity for RNA, mechanism |
642992 |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
initial interaction between RNA and the enzyme is characterized by a high enthalpy of association. The minimal RNA binding site of the enzyme is eight nucleotides. Upon RNA binding, the enzyme undergoes structural modifications, although the interaction does not significantly modify the stability of the protein |
677275 |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
mechanism |
642938 |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
non-uniform isomerization of the polymerase-primer complex with time consistent with a discontinuous (saltatory) translocation mechanism. Three distinct translocatory phases can be discerned: a -10(U)-binding site forward movement, a -27/-26(UU)-binding site jump to -10, then a -27/-26(UU)-binding site movement further downstream. Poly(A) tail elongation shows no apparent pauses during these isomerizations |
674824 |
2.7.7.19 | ATP + RNAn = diphosphate + RNAn+3'-A |
SN2-in-line-mechanism |
642984 |