1.8.4.10 | AMP + sulfite + thioredoxin disulfide = 5'-adenylyl sulfate + thioredoxin |
the reaction catalyzed by APR can be divided into three steps. In the first step, APS binds to the protein, and a reductive transfer results in sulfite bound to the active-site cysteine in a stable reaction intermediate. In the second step, free sulfite is released by the action of free thioredoxin. In the third step, the thioredoxin is regenerated by reduction with thioredoxin reductase |
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