EC Number |
Reaction |
Reference |
---|
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
enzyme forms a charge-transfer Michaelis complex with sarcosine, kinetics of formation of the Michaelis charge transfer complex can be directly monitored at 5°C. No redox intermediate is detectable during sarcosine oxidation |
672168 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
L-proline is the ionizable group in the ES complex. Y317 may play a role in substrate activation and optimizing of binding |
672010 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
mechanism |
392386, 392389, 392400, 392401, 392405, 392406 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
mechanism suggested |
685219, 685282, 687443 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
modified ping-pong-mechanism in which oxygen reacts with EredP prior to the dissociation of the imino acid product |
392408 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
molecular dynamics investigation by random acceleration molecular dynamics simulations with an ensemble made of the bacterial monomeric sarcosine oxidase (2GF3), O2, and the inhibitor furoic acid to mimic sarcosine |
742252 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
molecular dynamics simulation study for the heterotetrameric sarcosine oxidase-dimethylglycine complex gives insight to understand the dynamics of the enzyme. A cluster analysis on the small rectangular cells with high water probabilities results in the detection of eleven water channels. CH1, CH2, CH3, and CH4 correspond to the tunnels from the large cavity in the previous study. CH6, CH7, and the combined CH8 and CH9 are narrow channels from the large cavity. CH5 and the combined CH10 and CH11 are not connected with the cavity. No permeation of water molecules between the channels is found except in the combined channels. The results of the present analysis are consistent with the selective transport hypothesis |
742088 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
ping-pong bi-bi mechanism |
392388 |
1.5.3.1 | sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 |
the simulation method of Markovian milestoning molecular dynamics simulations is used to compute the entry and exit kinetics of O2 in the enzyme. The rate of flavin oxidation by O2 is likely not strongly limited by diffusion from the solvent to the active site. The predicted faster entry and slower exit of O2 for the bound state indicate a longer residence time within the enzyme, increasing the likelihood of collisions with the flavin isoalloxazine ring, a step required for reduction of molecular O2 and subsequent reoxidation of the flavin |
742971 |