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Reaction
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an acyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = an (11E)-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
a single enzyme may be responsible for the formation of both (Z)- and (E)-11-tetradecanoic acids. It is also possible that two mechanistically identical discrete enzymes are involved in each desaturation; reaction involves a first slow, isotope-sensitive C11-H cleavage, with probable formation of an unstable intermediate, followed by a second fast C12-H bond removal
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