EC Number |
---|
7.4.2.3 | - |
7.4.2.3 | affinity purification |
7.4.2.3 | by ion exchange, ATP-agarose affinity and hydroxyapatite chromatographies |
7.4.2.3 | isolation of mitochondria from Saccharomyces cerevisiae cells recombinantly expressing His-tagged mtHsp70, and further purification by nickel affinity chromatography |
7.4.2.3 | purified on nickel-Sepharose, by ion exchange on a Mono Q column, and by gel filtration |
7.4.2.3 | recombinant C-terminally His-tagged enzyme from Triticum aestivum germ lysates by nickel affinity chromatography |
7.4.2.3 | recombinant His-tagged full-length enzyme and isolated ATPase domain from Escherichia coli by nickel affinity chromatography, ammonium sulfate fractionation, anion exchange chromatography, and gel filtration |
7.4.2.3 | recombinant His-tagged mtHsp70 |
7.4.2.3 | recombinant protein |