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Search Purification (Commentary)

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Results 1 - 6 of 6
EC Number Purification (Commentary) Reference
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9- 1298, 1302, 1305, 675446
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9method that includes chromatography by a Talon resin 651400
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9method that includes DEAE-Trisacryl M and hydroxyapatite-Ultrogel chromatography. Large-scale purification using 2-mercaptoethanol as a reducing agent, results in 200 mg of purified enzyme from 54 l of culture, adducts with one or two molecules of 2-mercaptoethanol are formed. Replacement of 2-mercaptoethanol by dithiothreitol gave 162 mg of enzyme from 36 l of culture yielding no clusters with the reducing agent. 653712
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9recombinant His6-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography and dialysis 746203
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9recombinant protein 714367
Show all pathways known for 6.3.2.9Display the word mapDisplay the reaction diagram Show all sequences 6.3.2.9recombinant recombinant GST-tagged MurD from Escherichia coli by glutathione affinity chromatography, His-tagged MurD from Mycobacterium smegmatis by nickel affinity chromatography 690835
Results 1 - 6 of 6