EC Number |
---|
6.3.1.5 | - |
6.3.1.5 | native enzyme to homogeneity |
6.3.1.5 | Ni HiTrap column chromatography, and Superdex-200 gel filtration or Superdex-75 gel filtration |
6.3.1.5 | Ni-NTA Superflow resin chromatography |
6.3.1.5 | recombinant as His-tagged enzyme from Escherichia coli |
6.3.1.5 | recombinant enzyme from Escherichia coli, over 95% purity |
6.3.1.5 | recombinant His-tagged enzyme and recombinant thioredoxin-His6-fusion protein from Escherichia coli and insect cells, the His-tag is cleaved off afterwards |
6.3.1.5 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
6.3.1.5 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) star pLysS by nickel affinity chromatography, tag cleavage by a protease, another step of nickel affinity chromatography, and gel filtration |
6.3.1.5 | recombinantly overexpressed enzyme, to homogeneity |