EC Number   |
|---|
    6.1.1.7 | - |
| 6.1.1.7 | Ni-NTA agarose column chromatography, and gel filtration |
| 6.1.1.7 | nickel-chelating Sepharose resin column chromatography |
| 6.1.1.7 | partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities |
| 6.1.1.7 | purified by Ni-NTA chromatography and SDS gel electrophoresis |
| 6.1.1.7 | recombinant full-length AlaRS and AlaRS domains from Escherichia coli strain BL21 by anion exchange chromatography followed by hydrophobic interaction and adsorption chromatography, respectively |
| 6.1.1.7 | recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 6.1.1.7 | recombinant His6-tagged full-length enzyme and C-terminal domain C-Ala from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, followed by tag cleavage through TEV protease, tag and TEV protease are removed by another turn of nickel affinity chromatography, and ultrafiltration and dialysis |
| 6.1.1.7 | recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis |
| 6.1.1.7 | recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration |
