EC Number   |
|---|
    5.3.1.14 | - |
| 5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography |
| 5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose ion-exchange chromatography |
| 5.3.1.14 | His-Trap column chromatography |
| 5.3.1.14 | His-Trap HP column chromatography, and gel filtration |
| 5.3.1.14 | native enzyme by anion exchange, hydrophobic interaction, and again anion exchange chromatography to apparent homogeneity, recombinant His6-tagged enzyme 3.87fold from Escherichia coli strain JM109 by nickel affinity chromatography to homogeneity |
| 5.3.1.14 | Ni-NTA column chromatography |
| 5.3.1.14 | recombinant |
| 5.3.1.14 | recombinant His-tagged enzyme |
| 5.3.1.14 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis |
