EC Number   |
|---|
    5.1.1.7 | - |
| 5.1.1.7 | by immobilized nickel metal-affinity chromatography using 5 ml HisTrap HP columns and by gel filtration as His-tagged enzyme |
| 5.1.1.7 | by using nickel-chelate affinity chromatography |
| 5.1.1.7 | partial |
| 5.1.1.7 | protein is applied to a Ni21-primed chelating sepharose column, and DapF-containing eluate, fractions are dialysed and further purified by anion exchange chromatography on a HiTrap Q Sepharose FF column, representing a yield of 1 mg/L culture |
| 5.1.1.7 | recombinant His6-tagged enzyme from Escherichia coli strain SoluBL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, ultrafiltration, and gel filtration |
| 5.1.1.7 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS/pET15b by nickel affinity chromatography |
| 5.1.1.7 | recombinant seleno-methionine substituted wild-type and mutant enzymes from Escherichia coli strain B834(DE3) |
