EC Number   |
Reference |
|---|
    5.1.1.13 | - |
2075, 2076, 2077, 2078, 649759, 650892, 650894, 661647 |
| 5.1.1.13 | aspartate racemase is purified from the Scapharca broughtonii foot muscle by a procedure that includes sodium sulfate fractionation and blue sepharose column chromatography. The purified enzyme (225 mg) is digested at 25°C for 12 h with 80 mg of lysyl endopeptidase in a reaction mixture comprising 50 mM Tris-HCl, pH 8.0, containing 2 M guanidine hydrochloride, and the resulting peptides is resolved by reverse phase high performance liquid chromatography on a capcell Pac C-8 column (0.2 mm x 15 cm) using an acetonitrile gradient in 0.06% trifluoroacetic acid |
674359 |
| 5.1.1.13 | His-tagged aspartate racemase is purified on Talon metal affinity resin |
706558 |
| 5.1.1.13 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration |
746649 |
| 5.1.1.13 | recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography |
746724 |
| 5.1.1.13 | recombinant C-terminally His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
746724 |
| 5.1.1.13 | recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis anion change chromatography, and ultrafiltration |
747720 |
| 5.1.1.13 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
746669 |
| 5.1.1.13 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography |
748452 |
| 5.1.1.13 | recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21 and HEK-293T cells by nickel affinity chromatography and desalting gel filtration |
746719 |
