EC Number   |
|---|
   4.2.3.9 | - |
| 4.2.3.9 | His-bind Ni2+ column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration |
| 4.2.3.9 | mutant enzyme Y92C and Y92A |
| 4.2.3.9 | native and recombinant enzyme |
| 4.2.3.9 | Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration |
| 4.2.3.9 | Proteins are extracted from the inclusion bodies and purified following established protocols, each enzyme is pure as judged by SDS-gel electrophoresis |
| 4.2.3.9 | recombinant enzyme |
| 4.2.3.9 | using DE52 anion exchange resin, a methyl HIC, a Sephadex G-25, and a High Q column |
| 4.2.3.9 | wild-type and mutant enzymes |
