EC Number   |
|---|
   4.2.3.13 | affinity chromatography, chromatography on glutathione-Sepharose-4B column and anion-exchange column |
| 4.2.3.13 | CAD1-A isoenzyme |
| 4.2.3.13 | DEAE-Sepharose column chromatography |
| 4.2.3.13 | N-terminal fusion of 10x His-tag, purification by affinity chromatography under native and denaturing conditions |
| 4.2.3.13 | Ni-NTA affinity chromatography |
| 4.2.3.13 | Ni-NTA affinity column chromatography |
| 4.2.3.13 | Ni-NTA column chromatography |
| 4.2.3.13 | purification of a recombinant enzyme using histidine affinity chromatography |
| 4.2.3.13 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, diaylsis, and ultrafiltration |
| 4.2.3.13 | slurry homogenization and centrifugation |
