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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7-
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7ammonium sulfate precipitation followed by DEAE-Sephacel and Sephacryl S-200 column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7by several chromatographic steps, including QAE-cellulose, DEAE-cellulose, Sephadex and hydroxyapatite
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7enhancement of enzyme production by 92% is achieved by manipulation of the fermentation conditions (pH: 6.5, inoculum size of 0.5-1.0%, incubation temperature 30°C) and medium ingredients (replacement of dextrose by mannitol, soy peptone by chitin and ammonium sulfate by ammonium nitrate and supplementation of pyridoxine hydrochloride and glutaric acid)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7enzyme expressed in Escherichia coli as a fusion protein with GST at the N-terminus, one-step affinity purification
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7immobilization
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7immobilized metal ion affinity chromatography, ion exchange chromatography, gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7ion exchange chromatography
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7ion-exchange chromatography (SP-Sepharose), hydroxylapatite chromatography, mutant proteins by immobilized metal-chelate chromatography, His-tag is removed, further purification by ion-exchange chromatography
Display the word mapDisplay the reaction diagram Show all sequences 4.2.2.7large scale
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