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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.127-
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.127native enzyme from the inner membrane using subcellular fractionation including a sucrose-gradient centrifugation and ultracentrifugation at 150000 x g, anion exchange and hydrophobic interaction chromatography, and gel filtration, the detergent-mediated release of the protein from the membrane has irreversible inhibitory effects on the enzyme activity, spheroplast preparation
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.127native enzyme LinD out of crude extracts of strain 65Phen with a yield of 0.02% of the total soluble extract
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.127recombinant enzyme from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography, and gel filtration
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