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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.103- 393828
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.103native enzyme 14.6fold by ammonium sulfate fractionation followed by two steps of hydrophobic interaction chromatography 715552
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.103recombinant N-terminally His-tagged wild-type and mutant ICHs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His-tag through thrombin, and benzamidine affinity chromatography. The final recombinant ICH protein has three vector-derived amino acids at the N terminus, GSH 715542
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.103recombinant wild-type and mutants from Escherichia coli 652335
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