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Purification (Commentary)
4.1.3.44
ammonium sulfate precipitation, butyl Sepharose column chromatography, and HiTrap chelating column chromatography
4.1.3.44
ammonium sulfate precipitation, HiTrap chealting column chromatography, and butyl Sepharose column chromatography
4.1.3.44
purification and reconstitution of recombinant protein
4.1.3.44
purified anaerobically and reconstituted with Fe and S. The reconstituted protein is brown and has an absorbance spectrum that displays a characteristic shoulder at 400 nm
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