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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.8- 688342, 712237
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.8ammonium sulfate precipitation, DEAE-Sephacyl, hydroxylapatite 209767
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.8DEAE-Sepharose column chromatography, Mono Q GI column chromatography, and Superdex 200 gel filtration 678457
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.8hydroxyapatite column chromatography (native enzyme) and glutathione Sepharose column chromatography (recombinant enzyme). The activity of the enzyme purified at room temperature is 1.4times that of the enzyme purified at 4°C 734642
Display the word mapDisplay the reaction diagram Show all sequences 3.5.5.8native enzyme from strain THI115, and recombinant enzyme from Echerichia coli strain BL21(DE3) by three steps of butyl-resin chromatography, hydroxyapatite and ion exchange chromatography, or by 4 steps of butyl-resin chromatography and gel filtration 668637
Results 1 - 5 of 5