EC Number   |
|---|
   3.5.3.18 | - |
| 3.5.3.18 | anion exchange chromatography and gel filtration chromatography |
| 3.5.3.18 | by immobilized nickel-affinity chromatography and gel filtration |
| 3.5.3.18 | HisTrap column chromatography and HiTrap Q Sepharose column chromatography |
| 3.5.3.18 | Ni-NTA affinity resin chromatography and phenyl-Sepharose column chromatography |
| 3.5.3.18 | recombinant enzyme, partial |
| 3.5.3.18 | to more than 95% homogeneity |
| 3.5.3.18 | using Ni-NTA-agarose |
| 3.5.3.18 | wild type to more than 98% homogeneity, by Ni-NTA affinity chromatography, mutant required additional purification step, ion-exchange chromatography followed by affinity chromatography, mutant purified to more than 95% homogeneity |
