EC Number   |
|---|
   3.4.22.67 | - |
| 3.4.22.67 | native enzyme 14.91fold from rhizomes by ammonium sulfate fractionation, tert-butanol precipitation at 25°C and pH 7.0 using the interfacial precipitateand the aqueous phase, and ultrafiltration, method optimization |
| 3.4.22.67 | native enzyme from rhizomes by ammonium sulfate fractionation and dialysis |
| 3.4.22.67 | native enzyme from rhizomes by ammonium sulfate fractionation, anion exchange chromatography, SDS-PAGE, and gel filtration |
| 3.4.22.67 | native enzyme from rhizomes by saturation ammonium sulfate fractionation and anion exchange chromatography to homogeneity |
| 3.4.22.67 | partially purified 252fold with a recovery of 61%, ion exchange chromatography |
| 3.4.22.67 | purification via a three-phase partitioning system |
| 3.4.22.67 | ultrasonic-assisted liquid-liquid microextraction of the proteases from ginger and sodom apple using natural deep eutectic solvents, NADES. Selective partitioning of NADES-assisted microextraction yields more protease in NADES-enriched top phase. Maximum yield is achieved with 25% (v/v) of NADES, 15% (w/v) of source concentration and ultrasound temperature and time as 35°C and 10 min, respectively |
