EC Number   |
|---|
   3.4.22.14 | - |
| 3.4.22.14 | Act d 1 is purified from kiwifruit extracts by covalent chromatography using activated thiopropyl sepharose 6B |
| 3.4.22.14 | actinidin can be separated into six proteases, named KP1, KP2, KP3, KP4, KP5 and KP6 |
| 3.4.22.14 | Actinidin is purified from the soluble fraction of kiwi fruit by ion exchange chromatography on DE-52 and Mono-Q columns |
| 3.4.22.14 | ammonium sulfate precipitation and DEAE-Sephadex gel filtration |
| 3.4.22.14 | DEAE-Sephadex A-50 gel filtration and SP-Sephadex C-50 gel filtration |
| 3.4.22.14 | multiple forms |
| 3.4.22.14 | the enzyme fraction is precipitated from kiwifruit extract by 60% saturation of ammonium sulfate. The precipitate is redissolved in 50 mM citrate buffer (pH 5.5) and dialyzed overnight against this buffer. The dialyzate is loaded into a DEAE-Sepharose Fast Flow column, which pre-equilibrated with the same buffer. The adsorbed fractions are eluted with 0.0-1 M linear gradient of sodium chloride in the buffer |
