EC Number   |
Reference |
|---|
   3.2.2.29 | - |
691144, 692741, 692934, 693003, 694296, 694333, 694923, 727734, 731706, 751725 |
| 3.2.2.29 | cDNA sequence encoding human TDG (residues 112339) is subcloned into a pGEX4T-3 vector. SUMO-3-modified TDG112339 (SUMO-3TDG) is expressed bacterially using an Escherichia coli SUMOylation system by co-transforming the Escherichia coli strain BL21(DE3) with TDG112339/pGEX4T-3 and pT-E1E2S2 protein expression vectors |
693615 |
| 3.2.2.29 | glutathione Sepharose column chromatography |
731862 |
| 3.2.2.29 | GSTrap column chromatography |
750696 |
| 3.2.2.29 | HiTrap Q column chromatography |
749910 |
| 3.2.2.29 | Mono Q column chromatography as final chromatographic step |
751751 |
| 3.2.2.29 | nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration |
732585 |
| 3.2.2.29 | recombinant |
694423 |
| 3.2.2.29 | recombinant enzyme from NIH3T3 fibroblasts |
710145 |
| 3.2.2.29 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography |
710481 |
