EC Number |
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3.2.1.183 | - |
3.2.1.183 | neuC gene is cloned into an intein expression vector |
3.2.1.183 | purification from Escherichia coli results in 100 microgram/l pure and structurally intact protein. For insect cells, purification methods are established which result in up to 50 mg/l pure, soluble, and active protein |
3.2.1.183 | using a single ion exchange chromatographic step. The resulting protein is estimated to be 80% pure |
3.2.1.183 | using salmine sulfate precipitation and chromatography on phenyl-Sepharose, ATP-agarose, and Mono Q |