EC Number |
---|
3.2.1.157 | - |
3.2.1.157 | ammonium sulfate precipitation, butyl-Toyopearl column chromatography, heparin affinity column chromatography, and Superdex 200 gel filtration |
3.2.1.157 | native enzyme 10.3fold by gel filtration |
3.2.1.157 | native enzyme 31fold by gel filtration |
3.2.1.157 | native enzyme by hydrophobic interaction chromatography |
3.2.1.157 | Ni-Sepharose column chromatography, Source 15S column chromatography, gel filtration |
3.2.1.157 | purified by affinity chromoatography |
3.2.1.157 | recombinant enzyme from Escherichia coli strain BL21(DE3) culture supernatant by affinity chromatography |
3.2.1.157 | recombinant His-tagged enzyme from Brevibacillus choshinensis strain HPD31-SP3 by dialysis, nickel affinity chromatography, and gel filtration |
3.2.1.157 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |