EC Number   |
|---|
   3.2.1.156 | - |
| 3.2.1.156 | by using a Ni-NTA agarose column |
| 3.2.1.156 | by using a single-step HisTrap purification performed on a Atka fast protein liquid chromatograph. Protein yield: 140 mg recombinant enzyme per liter cell culture |
| 3.2.1.156 | Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography |
| 3.2.1.156 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL-21 Codon-Plus(DE3) RIL by metal affinity chromatography |
| 3.2.1.156 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography to homogeneity |
