EC Number   |
|---|
   3.1.3.75 | - |
| 3.1.3.75 | from solubilized inclusion bodies, ion-exchange chromatography (Q-Sepharose), dialysis, gel filtration |
| 3.1.3.75 | immobilized metal ion affinity chromatography (Ni2+) |
| 3.1.3.75 | Ni2+-Sepharose activated column |
| 3.1.3.75 | recombinant enzyme from Escherichia coli by chitin affinity chromatography |
| 3.1.3.75 | recombinant His-tagged wild-type and mutant fragments M19-C267 from Escherichia coli by nickel affinity chromatography to homogeneity |
| 3.1.3.75 | recombinant N-terminally His6-tagged PchP from Escherichia coli by nickel affinity chromatography |
| 3.1.3.75 | recombinant PchP from Escherichia coli, as an N-terminal fusion to intein or a histidine tag, in the folded state |
| 3.1.3.75 | recombinant PHOSPHO1 |
