EC Number   |
Reference |
|---|
   3.1.3.56 | - |
646374, 646376, 646377, 646379, 646380 |
| 3.1.3.56 | His-tag fusion protein expressed in Escherichia coli |
646400, 646402 |
| 3.1.3.56 | membrane associated enzyme |
646392 |
| 3.1.3.56 | partial purification of membrane bound and soluble enzyme |
646373 |
| 3.1.3.56 | partial purification of membrane bound enzyme |
646385 |
| 3.1.3.56 | partial purification of type I enzyme |
646386 |
| 3.1.3.56 | purification by a dual tag strategy: cDNA encoding human Type I Ins(1,4,5)P3 5-phosphatase is subcloned into a modified pMAL expression vector. This plasmid produces a recombinant protein in fusion with affinity tags located at its N-terminus, consisting in a maltose binding protein (MPB) and an octa-histidine stretch. The construction is transformed into Escherichia coli BL21 (DE3) expression strain. Yield: 18 mg of pure and stable enzyme starting from a 2 L |
682680 |
| 3.1.3.56 | recombinant enzyme using His-tag |
646396 |
| 3.1.3.56 | recombinant enzymes from Escherichia coli using His-tag |
646395 |
| 3.1.3.56 | recombinant His-tagged catalytic domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off |
665575 |
