3.1.1.45 | recombinant and wild-type trans-DLH are purified from cell extracts of Escherichia coli XL10-GOLD and Pseudomonas reinekei by subsequent anionic exchange, hydrophobic interaction, gel filtration and anionic exchange chromatography, the strep-tagged recombinant protein is purified using a Strep-Tactin Sepharose column |