EC Number   |
|---|
    2.7.9.3 | - |
| 2.7.9.3 | 150fold |
| 2.7.9.3 | DEAE-cellulose column chromatography, Sephacryl S-300 gel filtration, Heparin Toyopearl column chromatography, and hydroxylapatite column chromatography |
| 2.7.9.3 | DEAE-Sepharose chromatography is used after an ammonium sulfate fractionation step followed by phenyl-Sepharose and butyl-Sepharose chromatography |
| 2.7.9.3 | native enzyme from liver, by anion exchange chromatography, gel filtration, heparin affinity and hydroxyapatite chromatography |
| 2.7.9.3 | Ni-agarose column chromatography |
| 2.7.9.3 | on a Ni-chelating column, the His-tag is removed by thrombin digestion, further purification by gel-filtration |
| 2.7.9.3 | partial |
| 2.7.9.3 | recombinant C-terminally His6-tagged enzyme mutant Sephs2U60C from Escherichia coli Rosetta (DE3) pLysS by nickel affinity chromatography |
| 2.7.9.3 | recombinant from COS-7 cells as FLAG tagged enzyme |
