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EC Number	Purification (Commentary)
2.7.7.83	-
2.7.7.83	affinity chromatography (glutathione-Sepharose), GST part removed
2.7.7.83	anion exchange, phenyl interaction and gel filtration
2.7.7.83	recombinant His-tagged wild-type enzyme and mutant Y97N from Escherichia coli strain BL21-CodonPlus(DE3)-RIPL by nickel affinity chromatography and ultrafiltration
2.7.7.83	recombinant N-terminally GST-tagged wild-type and mutant AGX1 from Escherichia coli strain (DE3) pLysS by glutathione affinity chromatography, the tag is cleaved off by PreScission protease, followed by gel filtration, and ultrafiltration

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Release 2023.1 (January 2023)