EC Number |
---|
2.7.7.72 | - |
2.7.7.72 | native enzyme 11800fold by ammonium sulfate fractionation, anion exchange and hydroxylapatite chromatography, and tRNA affinity chromatography |
2.7.7.72 | purified by a combination of ammonium sulfate fractionation, gel filtration, and hydrophobic interaction chromatography (NTSFII) |
2.7.7.72 | purified by a combination of anion-exchange chromatography and hydrophobic interaction chromatography |
2.7.7.72 | recombinant C-terminally His-tagged enzyme from Escherichia coli |
2.7.7.72 | recombinant His-tagged HD domain from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography |
2.7.7.72 | recombinant N-terminally His-tagged mitochondrial CCase from Escherichia coli strain BL21 CodonPlus (DE3)-RP by nickel affinity chromatography and gel filtration to over 95% purity |