EC Number   |
|---|
   2.7.7.64 | - |
| 2.7.7.64 | ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE) |
| 2.7.7.64 | chelating chromatography (elution: 250 mM imidazole) followed by cleavage of N-terminal thioredoxin- and His-tag with thrombin and DEAE Sepharose FF chromatography |
| 2.7.7.64 | MonoQ HR 5/5 column chromatography |
| 2.7.7.64 | on a 1-ml HisTrap HP Ni2+-chelating, a HiPrep 26/10 desalting, and a 1-ml Q-Sepharose FF anion exchange column |
| 2.7.7.64 | recombinant |
| 2.7.7.64 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and gel filtration |
| 2.7.7.64 | recombinant His-tagged enzyme from Escherichia coli strain T7 Express by nickel affinity chromatography and ultrafiltration |
| 2.7.7.64 | recombinant His6-biotin-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography, tag cleavage via TEV protease, and dialysis |
| 2.7.7.64 | using Ni-NTA chromatography |
