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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68- 641771, 641822, 641823, 641831, 641832, 641833, 641834, 641840, 641842, 641844, 641849, 676808
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68amylose-resin column chromatography 759416
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68cytosolic enzyme type II enzyme and membrane-bound type I and type II enzyme 641825
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68glutathione-Sepharose chromatography 675019
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68glutathione-Sepharose column chromatography 759416
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Ni-NTA agarose column chromatography 759475
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Ni-NTA agarose column chromatography or glutathione-Sepharose column chromatography 759179
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68partial 641829
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS. 689477
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.68recombinan GST-fusion isozyme alpha from Escherichia coli 661757
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