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Search Purification (Commentary)

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Results 1 - 5 of 5
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.175native enzyme by heat treatment at 50°C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.175recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.175recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.175recombinant His-tagged enzyme from Mycobacterium smegmatis by nickel affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.175recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography
Results 1 - 5 of 5