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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.162-
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.162recombinant C-terminally His6-tagged wild-type and SeMet-labeled NahK residues 1-359 enzymes from Escherichia coli by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.162recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.162The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel.
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