EC Number   |
Reference |
|---|
    2.4.1.83 | - |
489426, 489428, 489432, 489433, 489434, 489438, 489439, 489442, 489456, 680037 |
| 2.4.1.83 | DEAE-cellulose column chromatography, gel filtration |
723410 |
| 2.4.1.83 | His-tag fusion protein purified using ProBond purification system, more than 97% pure |
703922 |
| 2.4.1.83 | one-step purification |
489455 |
| 2.4.1.83 | partial |
489447 |
| 2.4.1.83 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain C41(DE3) membranes by solubilization with 1% n-dodecyl-beta-D-maltoside in presence of 5% v/v glycerol, nickel affinity chromatography, followed by gel filtration and ultrafiltration. Mutant variant DELTA230-352 is prepared as two fractions, one purified from the membrane fraction (DELTA230-352 m) and one from the aqueous phase (DELTA230-352 s) |
757762 |
| 2.4.1.83 | solubilized with Triton X-100 and purified to homogeneity |
692257 |
