EC Number |
---|
2.3.1.B41 | - |
2.3.1.B41 | glutathione agarose column chromatography |
2.3.1.B41 | recombinant FLAG-tagged SIRT6 and Myc-tagged PER2 co-purify from HEK293 cells |
2.3.1.B41 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, reverse affinity chromatography, action exchange chromatography, and gel filtration |
2.3.1.B41 | recombinant N-terminally His-tagged human Sirt6 using from Escherichia coli by metal affinity chromatography, cation exchange chromatography, and gel filtration |