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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.64-
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.64ammonium sulfate, Biogel A, Sepharose 4B-agmatine
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.64Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.64frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE
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