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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258HiTrap SP column chromatography, and Superdex 75 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258nickel affinity column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant C-terminally His6-tagged enzyme RimI from Escherichia coli by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant GST-tagged full-length Naa50p from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and cleavage of the GST-tag by TEV protease, followed by ion exchange chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant GST-tagged hNaa50 and hNatA fromSf9 insect cells by affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant GST-tagged SpNatA and SpNaa50 from Escherichia coli by glutathione affinity chromatography and gel filtration, SpNaa50 maintains the ability to co-migrate with SpNatA in sizing buffer with NaCl concentration as high as 1 M
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.258recombinant hNaa15 mutants, hNaa50, and hNatA by affinity chromatography and gel filtration
Results 1 - 8 of 8