EC Number |
---|
2.3.1.133 | - |
2.3.1.133 | crude extracts from stem material from various down-regulated and control lines are assayed for extractable HCT enzyme activity |
2.3.1.133 | partial |
2.3.1.133 | recombinant enzyme |
2.3.1.133 | recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography |
2.3.1.133 | recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography |
2.3.1.133 | recombinant His-tagged HST from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis |
2.3.1.133 | recombinant N-terminally His6-tagged HCT from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography, dialysis, and cleavage of the tag by TEV protease, followed by gel filtration |
2.3.1.133 | soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography |
2.3.1.133 | tracheary elements are cultured |