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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the reaction diagram Show all sequences 2.1.1.282recombinant expression of His-tagged enzyme by affinity chromatography and desalting gel filtration
Display the reaction diagram Show all sequences 2.1.1.282recombinant expression of His-tagged wild-type and mutant enzymes to near homogeneity by 20 min heat treatment at 70°C and 65°C, respectively, followed by affinity chromatography and desalting gel filtration
Display the reaction diagram Show all sequences 2.1.1.282recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and heparin affinity chromatography, and dialysis
Display the reaction diagram Show all sequences 2.1.1.282recombinant wild-type and mutant enzymes
Results 1 - 4 of 4
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