EC Number   |
|---|
   2.1.1.103 | - |
| 2.1.1.103 | from E. coli, His-tag used for affinity chromatography |
| 2.1.1.103 | HisTrap column chromatography |
| 2.1.1.103 | Ni-NTA agarose column chromatography and Superdex 75 gel filtration |
| 2.1.1.103 | Ni-NTA column chromatography, gel filtration |
| 2.1.1.103 | Ni2+-nitrilotriacetic acid-agarose resin column chromatography and Superdex-200 gel filtration |
| 2.1.1.103 | nickel affinity column chromatography |
| 2.1.1.103 | nickel HisTrap column chromatography and Superdex 75PG gel filtration |
| 2.1.1.103 | nickel-affinity column chromatography and gel filtration |
| 2.1.1.103 | purification of the recombinant protein by affinity chromatography is found to be problematic and subject to losses in enzyme activity. The inclusion of DTT, BSA, and EDTA prevents the loss of enzyme activity during elution from a nickel ion matrix |
