1.8.5.3 | native Mo-DMSOR and engineered W-DMSOR, the latter from cell-free supernatant by ultracentrifugation at 150000 x g, ammonium sulfate fractionation, reduction with sodium dithionite followed by reoxidation with DMSO, hydrophobic interaction chromatography, and desalting gel filtration, followed by two different steps of anion exchange chromatography and ultrafiltration |