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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 1.5.7.1Display the reaction diagram Show all sequences 1.5.7.1-
Show all pathways known for 1.5.7.1Display the reaction diagram Show all sequences 1.5.7.1native enzyme 57.6fold from cytoplasm by anion exchange chromatography, hydrophobic interaction chromatography, ultrafiltration, and gel filtration, to homogeneity
Show all pathways known for 1.5.7.1Display the reaction diagram Show all sequences 1.5.7.1native enzyme aerobically 40fold by ultracentrifugation, anion exchange chromatography, gel filtration, ultrafiltration, hydroxyapatite chromatography, followed by again ultrafiltration and gel filtration, enzyme MetF copurifies with the other five proteins encoded in the unit in a hexaheteromeric complex with an apparent molecular mass in the 320-kDa range. Recombinant Strep-tagged enzyme MetF anaerobically from Escherichia coli strain C41(DE3) by avidine affinity chromatography and ultrafiltration
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