EC Number   |
Reference |
|---|
    1.5.1.34 | - |
484958, 484959, 484960, 484961, 484962, 484971, 484973, 484979, 484993, 484994, 484995, 484996, 484997, 484999 |
| 1.5.1.34 | ATCC 11299a |
484963 |
| 1.5.1.34 | cells centrifuged, washed with PBS (pH 7.4), resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5), disrupted by sonication, centrifugation, supernatant filtered (0.45 microm), applied to a column with nickel-NTA beads, eluted (50 mM Tris-HCl, pH 7.5), active fractions pooled, concentrated, and exchanged into 50 mM sodium phosphate, pH 6.8, ultrafiltration, applied to a cation-exchange chromatography HS20 column, eluted with salt gradient at pH 6.8, concentration by ultrafiltration, gel-filtration chromatography with Superdex 200 column in 50 mM Tris-HCl, pH 8.0, concentration of eluted fractions in 20 mM Tris-HCl, pH 8.0, by ultrafiltration |
695339 |
| 1.5.1.34 | homogenization of smooth muscle strips in 2-DE lysis buffer containing 8 M urea, 2 M thiourea, 65 mM DTT, 2% CHAPS, and protease inhibitor cocktail, centrifugation, supernatant used for 2-DE and MALDI-TOF/TOF-MS |
701144 |
| 1.5.1.34 | naphthoquinone affinity chromatography, 5'-AMP-Sepharose |
484978 |
| 1.5.1.34 | native enzyme and recombinant wild-type, W104F, A6V and D37I mutant enzyme |
484965 |
| 1.5.1.34 | native enzyme from fifth larval instar of p50 and ah09 strains, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration |
742313 |
| 1.5.1.34 | nickel-affinity column chromatography |
725004 |
| 1.5.1.34 | recombinant enzyme |
485009 |
| 1.5.1.34 | recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity |
654920 |
