EC Number |
---|
1.2.1.65 | by Ni2+ affinity chromatography followed by gel filtration |
1.2.1.65 | cell free extracts are prepared |
1.2.1.65 | His-tagged NahF purified by Ni-NTA chromatography |
1.2.1.65 | His-tagged NahV purified by Ni-NTA chromatography |
1.2.1.65 | native enzyme 28fold from cellfree extract by anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography, and gel filtration |
1.2.1.65 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography |