EC Number   |
|---|
   1.14.18.3 | - |
| 1.14.18.3 | by centrifugation and gel filtration |
| 1.14.18.3 | DEAE-Sepharose Fast Flow column chromatography, lysine agarose column chromatography, Sephacryl S-300HR gel filtration, QEA-Sephadex A-50 column chromatography, and Sephacryl S200 gel filtration, Superdex 200 gel filtration, or ammonium sulfate precipitation followed by Source 30Q column chroamtography |
| 1.14.18.3 | FPLC liquid chromatography and Mono Q HR 5/50 GL column chromatography |
| 1.14.18.3 | membrane-associated methane-oxidizing complex consisting of the particulate methane mono-oxygenase, pMMOH, and an unidentified component, assigned as a potential particulate methane mono-oxygenase reductase, pMMOR |
| 1.14.18.3 | MonoQ 10/100 GL column chromatography and Sephacryl S100 gel filtration |
| 1.14.18.3 | native enzyme from mebranes by solubilization with n-dodecyl beta-D-maltopyranoside, anion exchange chromatography, and ultrafiltration |
| 1.14.18.3 | native holoenzyme from isolated membranes by solubilization with detergent n-dodecyl beta-D-maltoside, ultrafiltration, and gel filtration |
| 1.14.18.3 | Ni2+-Sepharose Fast Flow column chromatography |
| 1.14.18.3 | optimization of solubilization and purification procedure for the hydroxylase component of membrane-bound enzyme, purification to homogeneity involves solubilization by dodexylbeta-D-maltoside, ion exchange chromatography and gel filtration, the purification includes the loss of the reductase component |
