EC Number |
---|
1.14.14.20 | on a Ni2+ column, to electrophoretic homogeneity |
1.14.14.20 | PheA1 and PheA2 expressed in recombinant Escherichia coli BL21 pLysS cells |
1.14.14.20 | recombinant His6-tagged PheA1 from Escherichia coli strain M15 |
1.14.14.20 | recombinant His6-tagged PheA2 from Escherichia coli strain M15 by nickel affinity chromatography |
1.14.14.20 | recombinant PheA1 from Escherichia coli by anion exchange and hydroxyapatite chromatography, followed by gel filtration |
1.14.14.20 | recombinant PheA2 from Escherichia coli by anion exchange and hydroxyapatite chromatography, followed by gel filtration |
1.14.14.20 | recombinant wild-type and selenomethionine-substituted PheA2 from Escherichia coli strain BL21(DE3)pLysS by protamine sulfate fractionation, hydrophobic interaction chromatography, dialysis, anion exchange chromatography, and preparative gel filtration |